rabbit h3k4me2 cell signaling 9725 chip Search Results


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Figure 5. <t>SREBP1</t> acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.
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Figure 5. <t>SREBP1</t> acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.
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Figure 5. <t>SREBP1</t> acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.
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Figure 5. <t>SREBP1</t> acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.
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Figure 5. <t>SREBP1</t> acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.
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Figure 5. <t>SREBP1</t> acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.
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Figure 5. <t>SREBP1</t> acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.
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Figure 5. <t>SREBP1</t> acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.
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Image Search Results


Figure 5. SREBP1 acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: SMYD3 Promotes Immune Evasion in Clear Cell Renal Cell Carcinoma via SREBP1-Mediated Transactivation of CD47.

doi: 10.1002/advs.202404200

Figure Lengend Snippet: Figure 5. SREBP1 acts as the pivotal downstream transcription factor of SMYD3 to promote immune evasion in ccRCC: A) Schematic illustration of the effects of SMYD3 on transcription factors in ccRCC cells. B) Dysregulation of these two TFs (SREBP1 and MLXIPL) in 3 histological subtypes of RCC from TCGA. KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; KICH: kidney chromophobe. C) SREBP1 transcript expression in multiple renal cancer studies from the GEO database. *p < 0.05, ***p < 0.001, Student’s t-test. D) SREBP1 mRNA expression in renal tumor tissues and paired normal tissues (n = 30 pairs). Student’s t-test. E) Representative IHC images of SREBP1 expression at the protein level in tumors compared with normal tissues from the TMA cohort. F) Changes in the mRNA expression level of CD47 upon SREBP1 knockdown in TK10 and 786-O cells, as determined by qPCR (n = 3). Data are presented as mean ± SEM of three experiments. **p < 0.01, ***p < 0.001, Student’s t-test. Data are presented as mean ± SEM.

Article Snippet: The antibodies used for ChIP analysis recognized the following targets: SMYD3 (sc-398085, Santa Cruz Biotechnology, Santa Cruz, CA, USA), SREBP1 (14088-1-AP, Proteintech), H3-K4 Me1 (#5326, Cell Signaling Technology, Danvers, MA, USA), H3-K4 Me2 (#9725, Cell Signaling Technology), H3-K4 Me3 (#9751, Cell Signaling Technology), and Sp1 (#9389, Cell Signaling Technology).

Techniques: Expressing, Knockdown